anti fn ab  (Innovative Research Inc)

Journal: International Journal of Molecular Sciences

Article Title: Lacticaseibacillus rhamnosus MS27 Potentially Prevents Ulcerative Colitis Through Modulation of Gut Microbiota

doi: 10.3390/ijms262311397

Figure Lengend Snippet: ( A ) The quantity percentage of CD45 + , CD11b + , and MHC II + cells in control group, n = 4. ( B , C ) The quantity percentage of CD45 + , CD11b + , and MHC II + cells in DSS and LR+DSS treatment groups; n = 4 for each treatment. All samples were processed using the same gating strategy. The quantity percentage of non-viable cells is on average, 8.357 ± 0.646 (%). ( D ) The quantity percentage values of CD45 + CD11b + MHC II + cells in three groups shown with violin plot. p < 0.05 indicates a significant difference. n = 4. ( E ) The quantity percentage values of CD45 + CD11b + F4/80 − MHC II + cells in three groups, n = 4. ( F ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + cells in three groups, n = 4. ( G ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + CD64 + cells in three groups, n = 4. ( H ) Serum CRP concentration in three groups; p < 0.05 means a significant difference, n = 8.

Article Snippet: The CytoPeak R1 3-laser 14-color flow cytometer (Dakewe Biotech Co., Ltd., Shenzhen, China) was used to detect the CD 45 (PerCP/Cyanine5.5 Anti-Mouse CD45, Cat#E-AB-F1136J, Elabscience, Wuhan, China), CD64 (PE/Cyanine7 Anti-Mouse CD64/FcγRI Antibody[X54-5/7.1], Cat#E-AB-F1186UH, Elabscience, China), F4/80 (Elab Fluor ® Violet 610 Anti-Mouse F4/80 Antibody[CI:A3-1], Cat#E-AB-F0995T, Elabscience, China), MHC II (APC Anti-Mouse MHC II Antibody[M5/114], Cat#E-AB-F0990UE, Elabscience, China), CD206 (FITC Anti-Mouse CD206/MMR Antibody[C068C2], Cat#E-AB-F1135C, Elabscience, China), CD19 (Elab Fluor ® Violet 450 Anti-Mouse CD19 Antibody, Cat#E-AB-F0986UQ, Elabscience, China), CD11b (PE CD11b anti-mouse, Cat#E-AB-F1081D, Elabscience, China) and CD68 (CoraLite ® Plus 750 Anti-Mouse CD68 Rabbit Recombinant Antibody, Cat#CL750-98029, Proteintech, Wuhan, China).

Techniques: Control, Concentration Assay

Journal: Biomedicines

Article Title: CD14 Is Involved in the Interferon Response of Human Macrophages to Rubella Virus Infection

doi: 10.3390/biomedicines10020266

Figure Lengend Snippet: During RuV infection and in the presence of exogenous IFN-β glycolytic reserve was lost in M-MΦ. ( A ) Analysis of surface expression of CD14, CD40, CD80, and CD86 in mock-, RuV UV -, and RuV-infected and exogenous IFN-β treated GM- and M-MΦ by flow cytometry after indicated incubation intervals. Relative surface marker expression after application of exogenous IFN-β (10 ng/mL) for 24 h was assessed by flow cytometry with the respective antibodies. Values ( n = 3) of the isotype-corrected MFI ± SD are shown. Statistical analysis was performed using ANOVA test and significance was calculated to the respective mock control. ( B ) M-MΦ (5 × 10 5 /mL) were ( BI ) mock-infected or infected with RuV UV or RuV or ( BII ) mock- and RuV UV -infected in the presence of IFN-β (10 ng/mL). Glycolysis was measured through extracellular flux analysis as real-time ECAR by the glycolysis stress test and successive injection of glucose to measure glycolysis and of oligomycin to measure glycolytic capacity and reserve. Data are shown in bars as the mean plus standard deviation (±SD) and were derived from three independent experiments with two wells per sample and experiment. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

Article Snippet: Flow cytometry analysis of markers expressed at the surface of MΦ was performed with direct dye labelled antibodies anti-CD14-APC mouse Ab (M5E2, BioLegend), anti-CD40-PerCp mouse Ab (Elabscience Biotechnology, Houston, TX, USA), anti-CD80-PE mouse Ab (L307, BD Pharmingen, San Jose, CA, USA), anti-CD86-pacific blue mouse Ab (IT2.2, BioLegend) and the respective isotype controls as described previously [ ].

Techniques: Infection, Expressing, Flow Cytometry, Incubation, Marker, Control, Injection, Standard Deviation, Derivative Assay